Standardization and kinetics of in vitro bovine blood lymphocyte stimulation with bovine rotavirus

Frequency of Bovine Lymphocyte Antigen DRB3.2 Alleles in Sarabi Cows

Whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune responses in bovine brucellosis.

A study was conducted to develop an in vitro whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune response in bovine brucellosis. A soluble antigen (BASA) prepared from killed cells of Brucella abortus 1119-3 was used. Cattle infected with B. abortus field strains, B. abortus 19 calfhood- and adult-vaccinated cattle, and nonexposed cattle were tested. Blood was dilut...

Inhibition of lymphocyte stimulation by bovine uterine proteins.

In vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

Subunit Vaccine Preparation of Bovine Rotavirus and Its Efficacy in Mice

Background: Rotaviruses (RV) are important viral diarrheal agents in calves. Vaccination is an optimum measure to prevent bovine rotaviruses (BRV) infection. However, little research on BRV VP7 vaccine has been done and currently there is no BRV vaccine. Objective: To prepare a subunit vaccine of BRV and investigate its efficacy. Methods: Total RNA was extracted from MA104 cells infected with b...